Objective To establish a high performance liquid chromatography (HPLC) method for the determination of paeoniflorin in a natural Chinese herbal medicine. Methods The chromatographic column was${\mathrm{C}}_{18}$column (${250}\mathrm{\;{mm}}\times$ ${4.6}\mathrm{\;{mm}},{5\mu }\mathrm{m}$), the mobile phase was acetonitrile -0.05% phosphoric acid aqueous solution $\left({{20}: {80}, V/V}\right)$, the flow rate was ${0.8}\mathrm{\;{mL}}/\mathrm{{min}}$, the column temperature was ${30}^{\circ }\mathrm{C}$, the detection wavelength is ${237}\mathrm{\;{nm}}$, and the injection volume was${10\mu }\mathrm{L}$. Results The linear range of paeoniflorin was ${24.68}\sim {246.8\mu }\mathrm{g}/\mathrm{{mL}}$, the linearity of paeoniflorin was good $\left({{r}^{2}= {0.9999}}\right.$, $n = 5$), the results of precision, stability and repeatability experiment were good, and the RSD values were less than $2\%$, the average recovery was ${102.0}\%$, RSD was ${1.49}\%\left({n = 9}\right)$. Conclusion The method is simple, convenient, easy to operate, and with strong specific, good separationand, good repeatability. It is suitable for the determination of paeoniflorin in natural Chinese herbal medicine, and provides a reference for the determination of paeoniflorin in compound preparations with Radix Paeoniae rubra as raw material.

The safety of university laboratories is a fundamental guarantee for promoting the continuous development of education and scientific research, and for teachers and students to carry out work and study smoothly. In China's vigorous promotion of the construction of "Double First Class” universities, the existing management methods and personnel configuration are unable to meet the current national requirements for laboratory safety management in universities. Building a scientific, comprehensive, practical, and easy-to-use laboratory safety management platform is an important issue that universities urgently need to study and solve. This article analyzes the necessity of building a laboratory safety management platform in universities under the current information technology background. Based on the actual work of Xinjiang University, a laboratory safety management system construction plan is proposed, and the system operation effect is analyzed to achieve systematic and intelligent management of the laboratory. It has played a positive role in supporting the cultivation of innovative talents and standardizing laboratory management, and has achieved certain results.

Based on the current status of university laboratory culture construction, this paper deeply analyzes the existing problems. Drawing on the concept of the management model of EHS (environment, health, safety) in management science, a generalized EHS model applicable to the management of university laboratories is proposed. Furthermore, an evaluation index system model (seven-force diagram) for laboratory safety culture construction is constructed based on the AHP (analytic hierarchy process) method, which can effectively enhance experimental safety and ensure standardized management of university laboratories.

Objective To study the application of SERS technique in the quantitative detection of antibiotic residues in poultry meat and evaluate its potential in food safety monitoring. Methods The SERS principle and enhancement factor calculation were introduced, and the influence of the shape and size of nanoparticles on SERS effect was analyzed experimentally and simulated. Different concentrations of antibiotic residues were detected through SERS, and the detection sensitivity and linear response were evaluated. Results SERS was reliable at${0.8}\mathrm{{nmol}}/\mathrm{L}$antibiotic concentration, the linear response range was${0.1}\sim {1000}\mathrm{{nmol}}/\mathrm{L}$, the${r}^{2}$was 0.999, and the enhancement factor was stable at${10}^{6}$. Conclusion SERS technology has high sensitivity and good linear response, which is suitable for the detection of antibiotic residues in food safety monitoring.

Objective Through the statistical analysis of the actual detection results of Down's disease screening and the pregnancy outcome of follow-up in Zhuhai, the health economics evaluation was carried out. Methods The data of 31967 pregnant women who underwent serum screening (SS) and non-invasive prenatal testing (NIPT) during the same pregnancy in Zhuhai City from 2018 to 2021 were retrospectively analyzed. The cost-benefit analysis was conducted according to 5 different screening strategies. Results Among 31,967 pregnancies, 38 were Down syndrome babies (including 3 pregnant women aged over 35 years), with a Down syndrome incidence of 1/841. Serological screening detected 1,649 cases of high risk of T21, of which 21 were true positives, with a positive predictive value of 1.27% and a detection rate of 55.3%; NIPT screening detected 49 cases of high risk of T21, 38 cases of Down syndrome babies, with a positive predictive value of 77.55% and a detection rate of 100%. The total cost generated by calculation strategy 4 was the lowest, about 27.44 million yuan. Conclusion NIPT can be used as primary screening test instead of serological screening method in prenatal Down's screening for secondary prevention and control of birth defects, which has great social and economic benefits.

Objective To improve the determination of free silica content in dust by pyrophosphoric acid heated by silicon carbide in order to ensure the accuracy of detection. Methods Analyze the influencing factors and controls during the experimental process, including the determination of dust sample composition, sample processing, preparation of pyrophosphate, control of heating temperature, hot filtration, ashing and weighing. Results By controlling and improving the influencing factors during the experimental process, measurement errors can be effectively avoided. Conclusion The importance of determining the characteristics of dust samples during the measurement process lies not only in the preparation of pyrophosphate and strict temperature control, but also in the accuracy of data analysis for quality control sample analysis.

This study aims to develop a rapid detection method based on multiplex real-time quantitative PCR technology for accurate identification and quantification of common pathogenic bacteria on face masks, to assess the hygienic condition of masks and control nosocomial infections. Methods Using TaqMan probe-based real-time fluorescence quantitative PCR as the core technology, specific primers and fluorescently labeled probes were designed and optimized for simultaneous quantification of specific gene sequences from three pathogens through one-tube multiplex detection. The experiment began by screening specific target genes and sequences from the NCBI database and designing primers and probes. Subsequently, quality testing of primers and probes was conducted, and standard samples were prepared through amplification of specific sequences and TA cloning. Finally, standard curves were established, and mask samples were tested. Results After optimizing experimental conditions, an efficient and stable multiplex real-time quantitative PCR detection system was successfully established. The detection efficiency of standard samples for three pathogens was evaluated at different concentration gradients. The detection data showed that the primers and probes had high specificity for target pathogens, and the amplification efficiency and signal intensity met experimental requirements. In the detection of mask samples, the method accurately quantified the genomic DNA copy numbers of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus hemolyticus, thereby assessing the degree of microbial contamination on masks. Conclusion The rapid detection method for pathogenic bacteria on masks based on multiplex real-time quantitative PCR technology demonstrates high sensitivity, specificity, and speed, providing strong technical support for clinical and public health

Objective This study aims to evaluate the stability and repeatability of digital pressure gauges in laboratory environments, to verify their measurement accuracy in different pressure ranges and performance in long-term use, and to provide scientific basis for the calibration and application of digital pressure gauges. Methods The experiment adopted a multi-point and multiple measurement method, selecting three different pressure points of 0.1,0.3,0.6 MPa for testing. Ten repeated measurements were taken at each pressure point to evaluate the repeatability of the digital pressure gauge. Meanwhile, through long-term measurements every three months for one year, observe the stability performance of the digital pressure gauge during long-term use. The experimental environment conditions are strictly controlled, with a temperature maintained at${\left({20}\pm 2\right)}^{\circ}\mathrm{C}$and a relative humidity between${40}\%$and${60}\%$. Results The standard deviations of the digital pressure gauge at the three pressure points were 0.10088 , 0.30057 and 0.60087, respectively. The standard deviations were small and the repeatability was good. At the same time, during long-term use, the measurement values at each measurement point have a relatively small change amplitude, and the changes in range and average values are within the allowable error range, indicating that it has good long-term stability. Conclusion Digital pressure gauges exhibit high repeatability and stability in laboratory environments, making them suitable for high-precision pressure measurement applications. This study provides a scientific basis for the calibration and practical application of digital pressure gauges, and also provides a reference direction for the research and improvement of related equipment in the future.

Objective To explore the application effect of C-reactive protein combined with blood routine and blood culture in the diagnosis of bacteremia. Methods In this study, 69 patients with suspected bacteremia examined from May 2023 to May 2024 were selected as the observation group. In order to more intuitively understand the application value of the three combined detection methods, 69 healthy patients in the same period were selected as the experimental group. Analysis of blood routine indexes, strain distribution in blood culture and C-reactive protein detection data in both participating groups. Results In the blood cultures. Results A total of 8 strains were detected in the blood culture of 69 suspected bacteremia patients in the observation group. Among the two strains with high detection rate, except gram-negative bacteria and gram-positive bacteria, the two strains with the highest detection rate were staphylococcus 25 strains. Escherichia coli 16 granules, and 16 E. coli with detection rate of${23.19}\%$. WBC$\left({{15.45}\pm {2.18}}\right)\times {10}^{9}/\mathrm{L}$, NEU$\left({{81.24}\pm {2.84}}\right)\%$, LYM$\left({{53.24}\pm {2.68}}\right)\%$, C-reactive protein levels (53.24±2.68) mg/L, WBC (6.45±1.28)×10${}^{9}$/L, NEU (62.41±2.34)%, LYM (26.45±2.18)%, and C-reactive protein levels$\left({{6.28}\pm {1.25}}\right)\mathrm{{mg}}/\mathrm{L}$. WBC, NEU, LYM and C-reactive protein levels in patients with suspected bacteremia in the observation group were significantly higher than those of healthy patients in the experimental group$\left({P <{0.05}}\right)$. Of the 69 suspected bacteremia, 66 were positive and 3 were negative. Among them, 63 cases were positive in blood culture, 58 cases were positive in blood routine test, 56 cases were positive in C-reactive protein test, and 66 cases were positive in C-reactive protein combined with blood routine and blood culture test. Among the four detection methods, C-reactive protein combined with blood routine and blood culture test were no different with the confirmed results. Conclusion In clinical diagnosis and detection of suspected bacteremia patients, C-reactive protein combined with blood routine and blood culture can maximize the accuracy of detection data, and the combined diagnosis of the three is of higher clinical value.